We are announcing a postdoctoral position at Lund University Diabetes Centre (LUDC).
Current lessons from genome-wide association studies (GWAS) of Type 2 diabetes (T2D) imply that most identified genetic variants associated with the disease are implicated in in b-cell function. Although many of these associations are firmly established, the mechanisms whereby they cause disease have remained unresolved. This hampers proper prevention of T2D and development of drugs for “Precision Medicine”.
Specific aim: Understand how genetic variants cause b-cell dysfunction in Type 2 Diabetes
The fellow will employ induced pluripotent stem cells (iPSC), as well as cultured clonal rodent and human b-cells, to examine the impact of genetic variation on relevant functional read-outs. The fellow will use genome editing tools (CRISPR/Cas9) complemented by RNA interference (RNAi), all established techniques in the laboratory, to manipulate genes of interest and change risk alleles. The consequences of these manipulations for insulin secretion, b-cell function and metabolism will be analyzed by an array of biochemical assays and novel imaging tools.
In depth analyses: The fellow will learn to gather data on how gene expression, epigenetic marks, metabolite levels, oxygen consumption and cellular function change when expression and/or the sequence of a T2D candidate gene has been manipulated. For the analyses, we will use our sequencing facility, metabolomics platform, the Seahorse XF24 flux analyzer of mitochondrial function, as well as real-time in vivo imaging of metabolic and cellular processes, including cellular second messenger systems.
A PhD (obtained less than 2 years ago) in a relevant area, e.g., diabetes/metabolic diseases, cellular imaging, cell culture and/or in vivo physiology is required. ONLY CANDIDATES FULFILLING THE ABOVE, WILL BE CONSIDERED.
Experience of stem cell biology and/or genome editing, as well as bioinformatics is beneficial. The candidates should be highly motivated and prepared to drive their own research, including mentoring of junior colleagues. Research in the Molecular Metabolism Unit is a team effort, requiring strong social skills and motivation to work in a collaborative fashion. A strong scientific track record (e.g., published work in international scientific journals and participation in international scientific meetings) will be of great merit.Continue reading
|Title||Postdoctoral position - Using iPS cells to analyze genetic variants controlling β-cell function in health and disease|
|Job location||Box 117, 221 00 Lund|
|Published||January 28, 2021|
|Job types||Postdoc  |
|Fields||Physiology,   Medical Imaging,   Genetics,   Cell Biology,   Endocrinology  |