We are announcing a postdoctoral position at Lund University Diabetes Centre (LUDC).
Physiological insulin release from the pancreatic b-cell is dependent on proper metabolism of nutrient fuels. Here mitochondrial metabolism plays a central role. In fact, failure of metabolism in b-cells may ultimately triggers the development of Type 2 Diabetes (T2D). Cellular metabolism generates coupling signals, which impact on ion channels and/or the exocytotic machinery in the b-cell. Understanding mitochondrial metabolism in insulin-producing cells is key to improve the generation of novel treatment strategies for the disease.
Specific aim: Understand how dysregulation of metabolism causes b-cell dysfunction in Type 2 Diabetes
The fellow will employ cultured clonal rodent and human b-cells, as well as primary human islets and animal models of diabetes to examine the impact of changes in b-cell metabolism due to altered metabolic state and/or disease. This will be complemented with use of genome editing tools (CRISPR/Cas9), RNA interference (RNAi) and virally mediated overexpression or knock down techniques, all established in the laboratory, to manipulate genes of interest. The consequences of these manipulations for hormone secretion, b-cell function and metabolism will be analyzed by an array of biochemical assays and novel imaging tools.
In depth analyses: The fellow will learn to gather data on metabolic parameters, for instance oxygen consumption rate and ATP/ATP ratios and how these parameters change when expression of a specific gene and/or metabolic state has been altered. For these, analyses, we will use our metabolomics platform, the Seahorse XF24 flux analyzer of mitochondrial function, as well as real-time in vivo imaging of metabolic and cellular processes, including cellular second messenger systems.
A PhD (obtained less than 2 years ago) in a relevant area, e.g., diabetes/metabolic diseases, cellular imaging, cell culture and/or in vivo physiology is required. ONLY CANDIDATES FULFILLING THE ABOVE, WILL BE CONSIDERED.
Experience in cell culture, in vivo work in mouse models, genome editing, imaging and bioinformatics is beneficial. The candidates should be highly motivated and prepared to drive their own research, including mentoring of junior colleagues. Research in the Molecular Metabolism Unit is a team effort, requiring strong social skills and motivation to work in a collaborative fashion. A strong scientific track record (e.g., published work in international scientific journals and participation in international scientific meetings) will be of great merit.Continue reading
|Title||Postdoctoral position - Detailed mapping of metabolic disturbances leading to β-cell dysfunction in Type 2 diabetes|
|Job location||Box 117, 221 00 Lund|
|Published||January 28, 2021|
|Job types||Postdoc  |
|Fields||Physiology,   Biochemistry,   Medical Imaging,   Genetics,   Cell Biology,   Endocrinology  |