Loïc Le Goff and Hervé Rigneault from the MOSAIC team at Institut Fresnel in Marseille (https://www.fresnel.fr/spip/spip.php?article1418) are seeking a postdoctoral candidate in biophysics to work on metabolic imaging and mechanobiology. The team has recently :
-Investigated the interplay between growth and mechanics in the growing Drosophila wing from a biophysics perspective using new in vivo imaging methods1–3.
-Developped a cutting edge expertize in label-free imaging of tissues based on coherent Raman imaging4,5, and is currently using it in the context of oncology in collaboration with Institut Paoli Calmette, Marseille.
In the proposed project, we will investigate biosynthetic and energetic metabolism in the context of normal and pathological growth regulation. In line with the state of the art in metabolic imaging6,7, coherent Raman imaging of deuterated compounds provides a unique opportunity to image metabolic fluxes in cultured cells or entire living embryos, at a subcellular resolution. We plan to use the technique in combination with state of the art fluorescence microscopy in the context of the mecanobiology of growth regulation in normal development (using drosophila wings as a model) and cancer (investigating breast cancer stem cells8,9 with our collaborators from Institut Paoli Calmette and IBDM in Marseille).
We are seeking for a candidate with a strong motivation for quantitative biology. He/she may be a cell or developmental biologist by training, with a desire to address biological questions using new imaging technologies, or a biophysicist willing to tackle biological questions hands on. To be eligible, the candidate must not hold a PhD from Aix Marseille University.
1. LeGoff, L. & Lecuit, T. Mechanical forces and growth in animal tissues. Cold Spring Harb. Perspect. Biol. (2016).
2. LeGoff, L., Rouault, H. & Lecuit, T. A global pattern of mechanical stress polarizes cell divisions and cell shape in the growing Drosophila wing disc. Dev. (2013).
3. Heemskerk, I., Lecuit, T. & LeGoff, L. Dynamic clonal analysis based on chronic in vivo imaging allows multiscale quantification of growth in the Drosophila wing disc. Dev. (2014).
4. Heuke, S., Sarri, B., Lombardini, A., Audier, X. & Rigneault, H. Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling. Opt. Lett. (2018). doi:10.1364/OL.43.004763
5. Heuke, S., Sarri, B., Audier, X. & Rigneault, H. Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies. Opt. Lett. (2018). doi:10.1364/OL.43.003582
6. Shi, L. et al. Optical imaging of metabolic dynamics in animals. Nat. Commun. (2018). doi:10.1038/s41467-018-05401-3
7. Chen, X., Grégoire, S., Formanek, F., Galey, J. B. & Rigneault, H. Quantitative 3D molecular cutaneous absorption in human skin using label free nonlinear microscopy. J. Control. Release (2015). doi:10.1016/j.jconrel.2014.12.033
8. Charafe-Jauffret, E. et al. Breast Cancer Cell Lines Contain Functional Cancer Stem Cells with Metastatic Capacity and a Distinct Molecular Signature. Cancer Res. (2009).
9. Ginestier, C. et al. ALDH1 Is a Marker of Normal and Malignant Human Mammary Stem Cells and a Predictor of Poor Clinical Outcome. Cell Stem Cell (2007). doi:10.1016/j.stem.2007.08.014Continue reading